cheminuminescent protein ladder for western blot

Western blotting: detection of the two unstained bands via the detection method used for the target protein.Please see the catalog or website for a complete listing of protein gels and Western blotting products. Western blot confirmation-detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein The ladder can be used with chemiluminescent.Fluorescent visualization-detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager.Wash the membrane 3 times with agitation for 10 minutes each in. Ensure the volume of the antibody solution is enough to fully cover the membrane. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › 1:5,000 (0.010.2 µg/mL) 1:5,000 (0.21.0 µg/mL) Incubate the membrane protein-side up in the primary antibody solution with agitation, for 1 hour at room temperature or overnight at 28☌.

We fused mouse and rabbit linear eptitopes (M&R LE) to generate a 15 kDa leader. IBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. Construction of M&R LE protein marker for Western blotting.

Cheminuminescent protein ladder for western blot